analytica-world.com Articles

Predictor(TM) hERG Fluorescence Polarization Assay Kit performed on the PHERAstar/PHERAstar Plus

The potential for cardiotoxic side-effects continues to challenge the development of small molecule based therapies. These intolerable side-effects are often precipitated by drug-induced long QT syndrome (LQT), which is often linked to blocking the ...

Detection of NADH and NADPH with the Omega's High Speed, Full UV/Vis Absorbance Spectrometer

Nicotinamide Adenine Dinucleotide (NAD+), reduced nicotinamide adenine dinucleotide (NADH), nicotinamide adenine dinucleotide phosphate (NADP+) and reduced nicotinamide adenine dinucleotide phosphate (NADPH) have been known to play vital roles in ...

"Green Chemstry" in the Laboratory

The term "green chemistry" is commonly used to describe chemical procedures which meet specific requirements with regards to the conservation of resources and protection of the environment. The objective is to avoid undesired by-products by ...

Particle Analysis: Quality Control of Catalysts with Digital Image Processing

Catalysts are widely used as an important auxiliary material in the chemical and petrochemical industry. They are able to accelerate chemical reactions by lowering the activation energy, i.e. they change reaction kinetics. Thus, using catalysts in ...

Polyolefins: Determination of Molecular Weight and Branching Structure by GPC/SEC

Polyethylene and Polypropylene are so-called Polyolefins. They are produced in large amounts every year. Depending on the process and catalyst used linear or branched polymers can be produced. In case of e. g. Polyethylene there are at least three ...

Molecular Probes� NanoOrange� Assay Performed on BMG LABTECH FLUOstar OPTIMA Microplate Reader

The field of proteomics has expanded dramatically in recent years with research on a whole host of organisms. Techniques such as two dimensional difference gel electrophoresis (2D DIGE) require the accurate quantitation of protein, as up to three ...

High sensitive detection of follistatin by Imperacer�: an initial study on the way to analytical gene-doping tests

For the understanding of interactions in the muscle-growth directing myostatin-pathway and therefore also for the establishment of high sensitive detection strategies for gene-doping, detailed studies of protein concentration levels in biological ...

Nature's Ingenuity Shows the Way

Up to 80 percent of our experience of our visual environment takes place via our visual perception. Without spatial vision, we would hardly be able to stay oriented. In recent decades, the neurosciences have gained many insights into the complex ...

Bradford Assay Performed on BMG LABTECH�s FLUOstar Omega with new Evaluation Software

Determining the protein concentration of samples is a necessary and often used method in biochemistry. Different colorimetric protein assays have been developed. The most commonly used methods are the Bradford assay, the Lowry assay and the BCA ...

Sensible and self-monitoring

For the generation of soft water are mostly used ion exchanging devices with generation of sodium chloride since the beginning of the Sixties. The generated soft water quality and soft water quantity are depending thereby on the static and dynamic ...

Efficient Transfection of Primary Human Skeletal Myoblasts Using FuGENE� HD Transfection Reagent

Transfection of cells is one of the main techniques used to influence gene expression. Most primary cells and human skeletal myoblasts (SkMC) in particular are very difficult to transfect, whereas for cell lines such as C2C12, many suitable ...

Enzymatic Protein Digest for Mass-spectrometric Analysis

The main focus in proteomic studies is on the identification of proteins in given biological samples. Proteins isolated and separated from a given sample (e.g., whole cell lysates, blood or tissue, protein complexes) by immunoprecipitation or ...

Real-Time Multiplex PCR of five Different DNA Targets Using the LightCycler� 480 System

One of the most interesting aspects of real-time PCR based on detection of fluorophoric-labeled oligonucleo�tides, such as Hydrolysis probes, and Molecular Beacons, is the possibility of being able to detect conveniently multiple targets in the same ...

High Pure PCR Cleanup Micro Kit - Novel Flexibility for Nucleic Acid Purification

Manual nucleic acid isolation with High Pure products is long approved by the scientific community. The product portfolio includes kits for isolation of genomic RNA and DNA, plasmid DNA as well as purification of reaction products. Key goal for all ...

Mutation Scanning of the Cytidine Deaminase Gene by High-Resolution Melting Curve Analysis Using the LightCycler� 480 System

Alexandre Evrard1,2, Caroline Raynal1,2, Jean-Christophe Boyer2, Lionel Le Gallic2, and Serge Lumbroso1,2 1Institut de G�nomique Fonctionnelle, D�partement d'Oncologie Mol�culaire et Cellulaire, Montpellier, France, 2Laboratoire de Biochimie, ...

Genotypes in Smokers: Correlations with Smoking Behavior

Smoking behavior is influenced by both genetic and environmental factors. Moreover, smoking initiation and smoking persistence have a heritability of at least 50%. A large body of work has been dedicated to associating multiple genetic markers of ...

Metagenomics Analysis Using the Genome Sequencer FLX System

The Genome Sequencer FLX System from 454 Life Sciences and Roche Applied Science is a versatile sequencing platform suitable for a wide range of applications, including de novo sequencing and assembly of genomic DNA, transcriptome sequencing, small ...

Experimental Workflow for Fast and Accurate Genotyping of Scrapie-resistant/sensitive Sheep

Several scientific studies support the claim that natural scrapie is associated with polymorphisms at three codons within the sheep prion protein (PrP) gene. Genotyping of these codons (codon 136 [alanine, valine], 154 [arginine, histidine], and 171 ...

The Universal ProbeLibrary - a Versatile Tool for Quantitative Expression Analysis in the Zebrafish

The teleost zebrafish is a widely used model organism for the study of developmental and disease-related processes in vertebrates, including humans. Understanding these processes at a molecular and cellular level frequently requires the estimation ...

Real-Time PCR Quantification of Plant miRNAs Using Universal ProbeLibrary Technology

MicroRNAs (miRNAs) are naturally occurring, highly conserved families of transcripts (18-25 nt in length) that are processed from larger hairpin precursors. Both plant and animal genomes encode miRNAs, and to date, there are over 4000 mature miRNA ...