Scientists adopt new strategy to find Huntington's disease therapies
Large, international NIH-supported study uses precision medicine to tackle neurological disorders
Scientists searched the chromosomes of more than 4,000 Huntington's disease patients and found that DNA repair genes may determine when the neurological symptoms begin. The results may provide a guide for discovering new treatments for Huntington's disease and a roadmap for studying other neurological disorders.
"Our hope is to find ways that we can slow or delay the onset of Huntington's devastating symptoms," said James Gusella, Ph.D., director of the Center for Human Genetic Research at Massachusetts General Hospital (MGH), Boston, and the corresponding author of the study. "This could be possible because we now have a list of clinically proven genetic factors that influence the disease."
Although studies in humans and animals have discovered clues as to how the disorder works, there are no effective treatments. For this study, the scientists tried a slightly different approach. They employed a novel application of Genome Wide Association Study analysis, a technique that scientists typically use to search for single letter changes to the DNA code on patients' chromosomes, which may increase or decrease their chances of having the disease. Here, the scientists already knew the disease-causing gene and so they used the technique to search for other changes that determine when the disease starts. They found that at least three sites were associated with symptoms appearing earlier or later than expected, two on chromosome 15 and one on chromosome 8.
The scientists used the patients' DNA and clinical information to study the age when movement problems began. Located on chromosome four, the Huntingtin gene is characterized by three letter repeats in the DNA code, called CAG-repeats. Disease-causing versions of the Huntingtin gene contain more than 35 CAG-repeats, which is higher than the six to 34 repeats found in normal versions. The greater number of CAGs a patient has, the greater the chances that symptoms will appear earlier in life. Focusing on patients who had 40 to 55 CAGs, the scientists found that some developed the disease earlier than expected while others developed it later.
The scientists then analyzed the patients' chromosomes using gene chips that contain sites known for having single letter changes to the DNA code in normal human populations. After multiple rounds of searching they found that two sites on chromosome 15 were strongly associated with disease onset. One site was associated with hastening the disease by, on average, just over six years; another site was associated with slowing the disease by about a year and a half. They also found that a site on chromosome 8 was associated with earlier disease onset by nearly one and a half years.
Although the scientists did not identify specific genes, they used several types of genome network analyses to show that disease onset may be controlled by genes that repair DNA, catalyze essential chemical reactions in cells, and assist with the division of mitochondria. In addition, they noted that a site within the code for MLH1, a DNA repair gene located on chromosome three, may be involved. Finally, the scientists showed that the sites on chromosomes 15 and 8 may be associated with the beginning of psychiatric and cognitive problems caused by the disease.
"These results are an important step toward developing new treatments for Huntington's disease. They help us understand how, through evolution, nature has learned to modify the disease-causing effects of Huntington's disease mutations," said Dr. Gusella. "As we study more patients we hope the information we obtain will soon reduce their suffering."
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