Single-cell study of tumor samples
Alan J. Simmons, Vanderbilt University
Ken Lau , Ph.D., and colleagues previously described a method for preparing single-cell suspensions from epithelial tissues – the type of tissue from which many diseases arise. Now, they have extended this analysis to formalin-fixed, paraffin-embedded (FFPE) specimens present in repositories of clinical patient samples.
They used the technique, called FFPE-DISSECT (disaggregation for intracellular signaling in single epithelial cells from tissue), to study human colon specimens. The analysis confirmed reduced differentiation in colorectal cancer compared to normal colon and revealed changes in the regulation of specific signaling pathways.
The approach, will enable detailed characterization of embedded human tumor samples to study how cellular heterogeneity contributes to tumor behavior and responds to treatment. The findings may guide personalized treatment strategies for cancer.
Original publication
Simmons, Alan J. and Scurrah, Cherié R. and McKinley, Eliot T. and Herring, Charles A. and Irish, Jonathan M. and Washington, M. Kay and Coffey, Robert J. and Lau, Ken S.; "Impaired coordination between signaling pathways is revealed in human colorectal cancer using single-cell mass cytometry of archival tissue blocks"; Science Signalling; 2016
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Topic World Cell Analysis
Cell analyse advanced method allows us to explore and understand cells in their many facets. From single cell analysis to flow cytometry and imaging technology, cell analysis provides us with valuable insights into the structure, function and interaction of cells. Whether in medicine, biological research or pharmacology, cell analysis is revolutionizing our understanding of disease, development and treatment options.
Topic World Cell Analysis
Cell analyse advanced method allows us to explore and understand cells in their many facets. From single cell analysis to flow cytometry and imaging technology, cell analysis provides us with valuable insights into the structure, function and interaction of cells. Whether in medicine, biological research or pharmacology, cell analysis is revolutionizing our understanding of disease, development and treatment options.